講師資料
Talks：
Deciphering ubiquitin-mediated regulation in plant circadian clock
Name：
李金美 (Chin-Mei Lee)
Position：
Assistant Professor
Affiliation：
Institute of Plant Biology
Global Agriculture Technology and Genomic Science Master Program
National Taiwan University
Email：
Photo：
Research Interests：
Plant circadian clock, protein ubiquitination, plant temperature responses, alternative splicing
Selected Publications：
1. Lee CM, Li MW, Feke AM, Liu W, Saffer AM, Gendron JM. (2019) GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex. Nat Commun. 10(1): 1-10.
2. Lee CM*, Feke AM*, Li MW, Adamcheck C, Webb K, Pruneda-Paz JL, Bennet EJ, Kay SA, Gendron JM. (2018) Decoys untangle complicated redundancy and reveal targets of circadian clock F-box proteins. Plant Physiol. 177(3): 1170-86.
3. Lee CM, Adamcheck C, Feke A, Nusinow DA, Gendron JM. (2017) Mapping protein–protein interactions using affinity purification and mass spectrometry. Plant Genomics: Methods and Protocols. (Humana Press) 231-249.
4. Lee CM, Thomashow MF. (2012) Photoperiodic regulation of the C-repeat binding factor (CBF) cold acclimation pathway and freezing tolerance in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 109(37):15054-9.
Abstract：
The circadian clock regulates approximately 24-hour rhythmicity of biological pathways to optimize organisms’ responses to the environment and thus increase their fitness. In plants, the clock governs growth, development, and stress resilience, and therefore biomass and quality of crops. The central theme driving the rhythms of the circadian clock across all kingdoms is transcription-translation feedback regulation accompanied by post-translational modifications. Protein ubiquitination mediated by E3 ubiquitin ligase (E3) and degradation by 26S proteasome has emerged as a conserved regulatory pathway in clock progression. However, due to the instabilities of the complex of E3 and its substrates, it remains largely unrevealed. We have developed an E3 ubiquitin ligase decoy strategy to stabilize the E3-substrate complex and overcome the challenge. This strategy was applied to co-immunoprecipitation to uncover the interactors and molecular mechanism of ZEITLUPE E3 ubiquitin ligases in regulating light inputs into the central circadian clock. We also utilized the E3 decoy strategy to establish an Arabidopsis E3 decoy library, which consists of around 25% of E3 in the Arabidopsis genome. We further set up a high-throughput yeast two-hybrid yeast screen platform to study the E3-clock regulators’ interacting network to gain a systematical view of the circadian clock and clock-regulated pathways in plants.