The mainstream method to identify and analyze bacterial is the 16S rRNA gene sequencing. The nearly 1500 bp 16S rRNA gene includes nine variable regions as known the highly conserved 16S sequence. BIOTOOLS CO., LTD. provides better taxonomic resolution using full-length 16S gene sequencing compared conventional V3-V4 sequencing. Nevertheless, our bioinformatic pipeline demonstrates taxonomic resolution at species and strain level. BIOTOOLS CO., LTD. also provides untargeted metabolomics for annotating metabolites that correlate with microbes and further accentuates the potential role of gut microbiota and metabolites as biomarkers to predict outcomes of several disease models.